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Human parvovirus B19 genotype study among the patients of Motol Univeristy Hospital
Dubišová, Mária ; Hubáček, Petr (advisor) ; Limberková, Radomíra (referee)
Parvovirus B19 is a common human pathogen that typically infects erythroid progenitors and causes hematological problems such as anemia and aplastic crises. The clinical presentation depends mainly on the immunological status of the patient. PVB19 can cause serious clinical disorders in immunocompromised patients after transplantation. More than 1500 samples from 90 patients who passed the HSCT in 2015 were tested for the presence of PVB19 in this work. This work describes the incidence of the virus and two typical periods of onset of infection in patients after the transplantation. Although several sources report the negative effect of PVB19 infection on the survival of allogeneic graft patients, this work did not confirm this assertion. Also, the results of this work suggest that allogenic grafts are not the main source for transmission, but that it is likely to be reactivated after long-term persistent or latent PVB19 infections. PVB19 is divided into 3 genotypes. Genotype 1 is the most widespread, genotype 2 is very rare in Europe for the last 10 years, and genotype 3 occurs mainly in tropical localities. This work as the first describes the distribution of genotypes in the Czech Republic. More than 130 samples from 125 PVB19 positive patients, stored in the Motol University Hospital from 2004...
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Detection and pathological impact of human Parvovirus B19 in the general population and in the immunocompromissed host
Dubišová, Mária ; Hubáček, Petr (advisor) ; Šmahelová, Jana (referee)
Parvovirus B19 is a non-enveloped single-stranded DNA virus, belonging to the Parvoviridae family. Despite parvoviruses are one of the smallest DNA viruses, some of them are able to infect humans. In general, most of humans experienced parvovirus B19 infection. In case of infection in healthy individual there are usually no complications. Most frequently infected are children and their symptomatic infection is known as fifth disease - erythema infectiosum (EI). However, serious complications may occur if infected person suffers from erythropoiesis disorders, weakened immunity or is pregnant. This thesis aimes the structure of the virus, the course of the infection, infectious diseases caused by parvovirus B19 and particular genotypes especially to pathogenesis of specific disorders. Key words: Parvovirus B19, single-stranded DNA virus, erythroid progenitor cells, Gb4, imunokompromissed host, anemia
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